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varies in a manner which is directly proportional to the applied magnetic eld. Early work on biomolecules used magnetic eld strengths of only approximately 40 MHz. Modern NMR spectrometers (Section 3.7.5) use much larger eld strengths (in the range 500 800 MHz) which give rise to larger E values and yield NMR spectra of much higher resolution. Combining Equations (3.38) and (3.2) shows that the electromagnetic frequency, , corresponding to a transition between these energy levels is: = 2 B0 sin h (3.39)

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Comparison of related protein sequences suggest that mutation of one amino acid residue for another is a major factor in the evolution of novel proteins. When such changes are deliberately made to proteins by site-directed mutagenesis, they often result in altered biological activity and stability. However, mutation rarely results in change in the overall fold of the protein. The most likely reason for this is delocalization of information specifying a particular fold over the whole protein rather than merely being speci ed by the sequence alone (Section 6.1.1). A mutagenesis study of the Arc repressor provides an interesting exception to this rule. Residue-by-residue differences in chemical shift show most changes concentrated between residues 9 and 14 Portion of NOESY spectrum showing cross-peaks between Leu-21 and other residues (numbered) in mutant Arc. NOEs shown as solid lines are not consistent with wild-type structure while those shown with dashed lines are.

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examinations routinely even if a clinical diagnosis can be made without them; however, this may change under cost containment of medicine or if litigious elements of society associate cancer cases with unnecessary radiation exposure due to overexposure from less-than-optimal techniques or retakes caused by operators with minimal or improper qualifications. The second element in radiation protection of patients is to ensure that quality radiographs that contain the requisite diagnostic information are obtained by using properly calibrated and maintained x-ray equipment that is kept in optimal operating condition by quality control procedures. Mammography examinations indicate a trend towards expected performance since they are now required to meet ACR guides for imaging objects in a breast phantom while keeping exposures within recommended guidelines. The remaining elements for patient protection from medical x-rays are based on good practices, some of which are: 1. 2. 3. Confine the field size to the regions being examined through proper collimation and shielding, especially for the reproductive regions. Use the maximum distance practicable between the x-ray source and the patient. Use the highest x-ray tube voltage practicable and use proper filtration of the x-ray beam to give the minimum absorbed dose consistent with producing a satisfactory radiograph. Pay particular attention to the film processor, especially ensuring accurate processing temperature and the quality and strengths of process chemicals. Use fast film/screen combinations and short exposure times. Plan all exposures carefully and minimize retakes.

FIGURE 9.20 Keep the cross of the Background Eraser cursor over the background you want to erase (top). If you inadvertently move the cross over the foreground, the foreground gets erased (bottom).

FIGURE 6.6. The essential components of Fizeau interferometric arrangement: S, point source. B. beam splitter to observe fringes in re ection OR ; L1, collimator; M1, M2, Fizeau mirrors, the wedge and separation very much exaggerated; L2, observing lens, which images the localized fringes between the mirrors at the observation plane OT .

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